SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Coser,Janaina; Boeira,Thaís da Rocha; Wolf,Jonas Michel; Cerbaro,Kamila; Simon,Daniel; Lunge,Vagner Ricardo.

Abstract Human papillomavirus (HPV) infection is common in sexually active women and viral persistence may cause intraepithelial lesions and eventually progress to cervical cancer (CC). The present study aimed to investigate epidemiological factors related to HPV infection and to evaluate viral persistence and CC precursor lesions frequencies in women from a city in the countryside of South Brazil. Three hundred women were recruited from a primary public health care clinic. The patients were interviewed and underwent sampling with cervical brushes for HPV-DNA detection/typing by a PCR-based assay and cytological analysis by Pap smear test. HPV was detected in 47 (15.7%) women. HPV infection was significantly associated with young age (<30 years) and low...

HBV epidemiology and genetic diversity in an area of high prevalence of hepatitis B in southern Brazil

Palavras-chave: HPV prevalence; HPV persistence; Cervical cancer; Epidemiology.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100061

Paoli,Juliana de; Wortmann,André Castagna; Klein,Mirelli Gabardo; Pereira,Vagner Reinaldo Zingalli Bueno; Cirolini,Adriana Maria; Godoy,Bibiane Armiliato de; Fagundes,Nelson Jurandi Rosa; Wolf,Jonas Michel; Lunge,Vagner Ricardo; Simon,Daniel.

ABSTRACT Background Hepatitis B virus (HBV) infection is a major public health problem in Brazil. HBV endemicity is usually moderate to low according to geographic regions, and high prevalence of this virus has been reported in people of some specific Brazilian counties, including those with a strong influence of Italian colonization in southern Brazil. Analysis of HBV diversity and identification of the main risk factors to HBV infection are necessary to understand hepatitis B epidemiology in these high prevalence regions in southern Brazil. Objective To investigate epidemiological characteristics and HBV genotypes and subgenotypes circulating in a specific city with high HBV prevalence. Methods A cross-sectional study was performed with 102 HBV...

Helicobacter pylori eradication: influence of interleukin-1beta -31 C/T polymorphism

Palavras-chave: HBV infection; Epidemiology; HBV genotypes; Risk factor; Transmission; Cross-sectional studies.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400294

Rech,Tássia Flores; Mazzoleni,Luiz Edmundo; Mazzoleni,Felipe; Francesconi,Carlos Fernando de Magalhães; Sander,Guilherme Becker; Michita,Rafael Tomoya; Nabinger,Débora Dreher; Milbradt,Tobias Cancian; Torresini,Ronaldo João Spinato; Simon,Daniel.

ABSTRACT Aim To analyze the influence of the -31 C/T polymorphism of the interleukin-1β gene on Helicobacter pylori eradication therapy success in patients with functional dyspepsia. Methods Functional dyspepsia was diagnosed according to the Rome III criteria. All patients underwent upper gastrointestinal endoscopy, and gastric biopsies were obtained at screening and 12 months after randomization (last follow-up visit). Urease test and histological examination were performed to define the H. pylori status. Patients received twice-daily amoxicillin, clarithromycin and omeprazole for 10 days. Genotyping of the interleukin-1beta -31 C/T polymorphism (rs1143627) was performed using polymerase chain reaction-restriction fragment length polymorphism. Results...

Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil

Palavras-chave: Helicobacter pylori; Dyspepsia; Interleukin-1beta; Polymorphism; Inflammation.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400311

Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo.

ABSTRACT Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries. Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil. Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis. Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)...

Interferon lambda 4 (IFNL4) gene polymorphism is associated with spontaneous clearance of HCV in HIV-1 positive patients

Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424

Alves,Camila Fernanda da Silveira; Grott,Camila Schultz; Lunge,Vagner Ricardo; Béria,Jorge Umberto; Tietzmann,Daniela Cardoso; Stein,Airton Tetelbom; Simon,Daniel.

Abstract Approximately one-third of the individuals infected with human immunodeficiency virus type 1 (HIV-1) are co-infected with hepatitis C virus (HCV). Co-infected patients have an increased risk for developing end-stage liver diseases. Variants upstream of the IFNL3 gene have been associated with spontaneous and treatment-induced clearance of HCV infection. Recently, a novel polymorphism was discovered, denoted IFNL4 ΔG > TT (rs368234815), which seems to be a better predictor of spontaneous clearance than the IFNL4 rs12979860 polymorphism. We aimed to determine the prevalence of the IFNL4 ΔG > TT variants and to evaluate the association with spontaneous clearance of HCV infection in Brazilian HIV-1 patients. The IFNL4 ΔG > TT genotypes were...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: IFNL4 genotypes; HIV/HCV co-infected patients; Spontaneous clearance.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300374

Low prevalence of human immunodeficiency virus and hepatitis C virus co-infection in a medium size city in southern Brazil

Librelotto,Carina Sperotto; Simon,Daniel; Ikuta,Nilo; Lunge,Vagner Ricardo.

Tipo: Info:eu-repo/semantics/other

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600689

Matrix metalloproteinase gene polymorphisms in patients with coronary artery disease

Dalepiane,Vanessa L.N.; Silvello,Daiane N.; Paludo,Crislaine A.; Roisenberg,Israel; Simon,Daniel.

Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of atherosclerosis, the pathology underlying the majority of coronary artery disease (CAD). In this study we tested the hypothesis that polymorphic variation in the MMP genes influences the risk of developing atherosclerosis. We analyzed functional polymorphisms in the promoter of the MMP-1, MMP-3, MMP-9 and MMP-12 genes in 183 Brazilian Caucasian individuals submitted to coronary angiography, of which 67 (37%) had normal coronary arteries (control group) and 116 (63%) had CAD (CAD patient group). The -1607 1G/2G MMP-1, -1171 5A/6A MMP-3, -1562 C/T MMP-9, -82 A/G MMP-12 polymorphisms were analyzed by PCR followed by restriction digestion. No significant differences were observed in...

Polymorphism in the promoter region of von Willebrand factor gene and von Willebrand disease type 1

Palavras-chave: Atherosclerosis; Coronary artery disease; Gene polymorphisms; Matrix metalloproteinases.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400001

Simon,Daniel; Bandinelli,Eliane; Roisenberg,Israel.

The -1185A/G polymorphism in the 5'-regulatory region of the von Willebrand factor (VWF) gene was associated with VWF plasma levels in a normal population. This study was undertaken to evaluate whether there is a relationship between this polymorphism and type 1 von Willebrand disease (VWD), a disorder characterized by a quantitative deficiency of VWF. The association between this polymorphism and plasma VWF levels in normal Brazilian individuals was also analyzed. Control subjects (n = 460) and type 1 VWD patients (n = 41) were studied. Polymerase chain reaction (PCR) amplification of the 864-bp VWF promoter region followed by AccII restriction-digestion was used to identify the -1185A/G genotypes. The -1185G allele frequency was 57% in normal individuals...

The association between adult-type hypolactasia and symptoms of functional dyspepsia

Palavras-chave: Von Willebrand factor; Promoter polymorphisms; Genetics; Von Willebrand disease.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400001

Wortmann,André Castagna; Simon,Daniel; Mazzoleni,Luiz Edmundo; Sander,Guilherme Becker; Francesconi,Carlos Fernando de Magalhães; Nabinger,Débora Dreher; Grott,Camila Schultz; Rech,Tássia Flores; Mazzoleni,Felipe; Lunge,Vagner Ricardo; Bona,Laura Renata de; Milbradt,Tobias Cancian; Silveira,Themis Reverbel da.

Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of...

Viral genotypes and human rs12979860 polymorphism of the IFNL3 gene in hepatitis C infected patients in Southern Brazil

Palavras-chave: Bloating; Dyspepsia; Gastrointestinal diseases; Lactose intolerance; Single nucleotide polymorphism.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100092